|
| Status |
Public on Feb 07, 2009 |
| Title |
4507_female_rat_liver_PB_24hr |
| Sample type |
RNA |
| |
|
| Source name |
Male and female Sprague-Dawley rats were dosed orally by gavage with PCN (100 mg/kg), PB (100 mg/kg), or vehicle (0.5% aqueous methylcellulose with 1% Tween 80
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley rats
|
| Treatment protocol |
Animals were housed individually in suspended wire cages and maintained on a diet of Certified Rodent Chow. Male and female rats (n= 5 per sex) were dosed orally by gavage with PCN (100 mg/kg), PB (100 mg/kg), or vehicle (0.5% aqueous methylcellulose with 1% Tween 80). Livers were collected at 6 and 24 h after a single dose or 24 h after 5 consecutive days of dosing (n = 5 animals per sex per time point), immediately flash frozen in liquid N2, and stored at approximately -70 C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the Trizole reagent (Invitrogen Corporation, Carlsbad, CA) and the Qiagen Rneasy mini RNA cleanup protocol (Qiagen, Valencia, CA)
|
| Label |
biotin-streptavidin-R-phycoerythrin
|
| Label protocol |
Double-stranded cDNA was synthesized, purified, and used to transcribe biotin-labeled cRNA with the BioArray High Yield Transcript Labeling Kit (ENZO Life Sciences, Farmingdale, NY)
|
| |
|
| Hybridization protocol |
Samples were hybridized according to the Affymetrix protocol
|
| Scan protocol |
scanned with the Hewlett Packard GeneArray Scanner
|
| Description |
gene expression data from phenobarbital or PCN treated rat liver
|
| Data processing |
Affymetrix scan data (.cel files) were imported into Rosetta Resolver for analysis
|
| |
|
| Submission date |
Feb 04, 2009 |
| Last update date |
Feb 06, 2009 |
| Contact name |
Hongzu Ren |
| E-mail(s) |
ren.hongzu@epa.gov
|
| Phone |
919-541-4576
|
| Organization name |
US Environmental Protection Agency
|
| Lab |
NHEERL Toxicogenomics Core
|
| Street address |
109 TW Alexander Dr
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27711 |
| Country |
USA |
| |
|
| Platform ID |
GPL85 |
| Series (1) |
| GSE14712 |
Xenobiotic-responsive Nuclear Receptors in Transcriptional Effects Upon Perfluoroalkyl Acid Exposure in Diverse Species |
|
