|
| Status |
Public on Apr 21, 2023 |
| Title |
6M_HBs-HepR, liver_rep5 |
| Sample type |
SRA |
| |
|
| Source name |
liver tissue
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
tissue: liver
time: 6m
treatment: HBs-HepR mice adoptively transferred with HBsAg+ hepatocytes from HBs-Tg mice
|
| Treatment protocol |
HBs-HepR mice was adoptively transferred with HBsAg+ hepatocytes from HBs-Tg mice for reconstruction, and B6-HepR mice was adoptively transferred with wildtype hepatocytes from C57BL/6J mice for reconstruction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
liver tissue were harvested, flash frozen in liquid nitrogen, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
6M_HBs-HepR_5
|
| Data processing |
Sequenced reads were mapped to GRCm38 whole genome using hisat v2.1.0 with parameters --threads 28 --dta -t
Read counts were computed using Rsubread using default settings
Genome_build: GRCm38
Supplementary_files_format_and_content: tab-delimited text files include raw counts for each Sample
|
| |
|
| Submission date |
May 03, 2019 |
| Last update date |
Apr 22, 2023 |
| Contact name |
Xiaolei Hao |
| Organization name |
Institute of Immunology, USTC
|
| Street address |
huangshan Road 443
|
| City |
Hefei |
| State/province |
Anhui |
| ZIP/Postal code |
230027 |
| Country |
China |
| |
|
| Platform ID |
GPL21273 |
| Series (2) |
| GSE130668 |
A novel mouse model of hepatocellular carcinoma mediated by hepatitis B virus-specific CD8+ T cells [bulk RNA-seq] |
| GSE130945 |
A novel mouse model of hepatocellular carcinoma mediated by hepatitis B virus-specific CD8+ T cells |
|
| Relations |
| BioSample |
SAMN11569404 |
| SRA |
SRX5784297 |
