|
| Status |
Public on Dec 31, 2009 |
| Title |
Nucleus Accumbens Shell, time from last drinking episode (hrs):24, ethanol-62 |
| Sample type |
RNA |
| |
|
| Source name |
Nucleus Accumbens Shell, time from last drinking episode (hrs):24, ethanol-62
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: P rat
tissue: Nucleus Accumbens Shell
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol followed by Qiagen RNeasy mini kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix 2-cycle protocol from 25 ng total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on RGU34A Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the standard Affymetrix protocol.
|
| Description |
exposed to limited access ethanol
time from last drinking episode (hrs):24
Gene expression data from brain nucleus accumbens shell
|
| Data processing |
The data were analyzed with RMA
|
| |
|
| Submission date |
Mar 26, 2009 |
| Last update date |
Mar 26, 2009 |
| Contact name |
Mark William Kimpel |
| E-mail(s) |
mkimpel@iupui.edu
|
| Organization name |
Indiana University
|
| Department |
Psychiatry
|
| Lab |
W.J. McBride
|
| Street address |
15032 Hunter Court
|
| City |
Westfield |
| State/province |
IN |
| ZIP/Postal code |
46074 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE15407 |
The Nucleus Accumbens Shell and Central Nucleus of the Amygdala of Alcohol-Preferring Rats Following Binge-Drinking |
|
