|
Status |
Public on Dec 31, 2009 |
Title |
Nucleus Accumbens Shell, time from last drinking episode (hrs):1, water-18 |
Sample type |
RNA |
|
|
Source name |
Nucleus Accumbens Shell, time from last drinking episode (hrs):1, water-18
|
Organism |
Rattus norvegicus |
Characteristics |
strain: P rat tissue: Nucleus Accumbens Shell
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using Trizol followed by Qiagen RNeasy mini kit.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix 2-cycle protocol from 25 ng total RNA (Expression Analysis Technical Manual, Affymetrix).
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|
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on RGU34A Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
Scan protocol |
GeneChips were scanned using the standard Affymetrix protocol.
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Description |
no ethanol time from last drinking episode (hrs):1 Gene expression data from brain nucleus accumbens shell
|
Data processing |
The data were analyzed with RMA
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|
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Submission date |
Mar 26, 2009 |
Last update date |
Mar 26, 2009 |
Contact name |
Mark William Kimpel |
E-mail(s) |
mkimpel@iupui.edu
|
Organization name |
Indiana University
|
Department |
Psychiatry
|
Lab |
W.J. McBride
|
Street address |
15032 Hunter Court
|
City |
Westfield |
State/province |
IN |
ZIP/Postal code |
46074 |
Country |
USA |
|
|
Platform ID |
GPL1355 |
Series (1) |
GSE15407 |
The Nucleus Accumbens Shell and Central Nucleus of the Amygdala of Alcohol-Preferring Rats Following Binge-Drinking |
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