|
| Status |
Public on Mar 01, 2020 |
| Title |
052_OPU: Granulosa cells_36h_rFSH_GNRHa |
| Sample type |
RNA |
| |
|
| Source name |
Granulosa cells collected 36 hours after induction of ovulation in a patient treated with rFSH, ovulation induced with GNRHa
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: granulosa cells isolated from aspirated follicular fluid
ovulation time point: 36h
treatment: rFSH
ovulation induction: GNRHa
|
| Treatment protocol |
Patients were treated with individually dosed rFSH (Puregon®, MSD, Denmark) or hMG (Menopur®, Ferring, Denmark) starting on day 2-3 of the menstrual cycle. From stimulation day 5-6 a GnRH antagonist (Ganirelix 0,25 mg (Fyremadel®, SUN pharma, Netherlands)) was administered daily. When at least three follicles reached ≥17 mm in diameter, an ovulation trigger (hCG (6500 IU (Ovitrelle®, Merck Serono, Germany) or GNRHa (buserelin 0,5 mg (Suprefact®, Sanofi-Aventis)) was administered and oocyte pick up was planned for 36 hours later.
|
| Growth protocol |
Granulosa cells were isolated from aspirated follicular fluid with a pipette as blood clots and vessels were avoided. The cells were washed through a 4-well dish containing PBS and 0,1% PVA, transferred to a cryotube with minimal PBS, snap frozen in liquid nitrogen and stored at -80⁰C until RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The Arcturus PicoPure® RNA isolation kit (Applied Biosystems, CA, USA) was used to isolate total RNA from GC according to the manufacturer’s instructions
|
| Label |
biotin
|
| Label protocol |
Phycoerytrin conjugated streptavidin
|
| |
|
| Hybridization protocol |
RNA was processed using Clariom™ D Pico Assay (Applied Biosystems, Thermo Fisher, USA) according to the manufacturer’s protocol, using the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Arrays were scanned in the Affymetrix GeneArray 3000 7G scanner to generate fluorescent images. Cell intensity files (.CEL files) were generated in the GeneChip Command Console Software (AGCC, Affymetrix, Thermo Fisher, USA).
|
| Description |
Gene Expression data from human granulosa cells, collected from a patient undergoing fertility treatment 36 hours after induction of ovulation
052_OPU
|
| Data processing |
The 83 raw CEL-files were imported into Transcriptome Analysis Console (TAC 4.0.1, applied Biosystems, Thermo Fisher Scientific, MA, USA) . Normalization and summarization were performed by the SST-RMA (Robust Multichip Analysis) approach.
|
| |
|
| Submission date |
Jul 05, 2019 |
| Last update date |
Mar 02, 2020 |
| Contact name |
Liv la Cour Poulsen |
| E-mail(s) |
livlacour@hotmail.com
|
| Organization name |
Zealand University Hospital
|
| Department |
Gynaecology and Obstetrics
|
| Street address |
Lykkebækvej 14
|
| City |
Køge |
| ZIP/Postal code |
4600 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE133868 |
Granulosa cell expression data from five different time points before and up to 36 hours after induction of ovulation in women receiving fertility treatment |
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