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| Status |
Public on Nov 01, 2019 |
| Title |
HCC827_AKT1 |
| Sample type |
SRA |
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| Source name |
HCC827 cell line, NSCLC
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| Organism |
Homo sapiens |
| Characteristics |
cell line: HCC827
treatment: AKT1
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent. RNA was prepared by using Ribo-Zero Magnetic Kit (rRNA-depleted RNA). Illumina TruSeq Stranded mRNA Kit was used for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
Bustard software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to HG38 whole genome using bowtie2.
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated by using HTseq.
Genome_build: HG38
Supplementary_files_format_and_content: Tab-delimited text files include RPKM values for each samples were attached.
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| Submission date |
Sep 05, 2019 |
| Last update date |
Nov 02, 2019 |
| Contact name |
Han Tian |
| E-mail(s) |
tianhan@mail2.sysu.edu.cn
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| Phone |
86-20-87333587
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| Organization name |
Sun Yat-sen University
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| Department |
Zhongshan School of Medicine
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| Street address |
No.74, Zhongshan Er Road
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| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510080 |
| Country |
China |
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| Platform ID |
GPL24676 |
| Series (1) |
| GSE136904 |
Strand specific Sequencing of myr-Akt1 induced transcriptomes in NSCLC cell lines |
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| Relations |
| BioSample |
SAMN12698164 |
| SRA |
SRX6804715 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM4061726_HCC827_AKT1.txt.gz |
259.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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