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Status |
Public on Dec 17, 2019 |
Title |
EA0996: Newcastle Early Arthritis Clinic number 996 |
Sample type |
genomic |
|
|
Source name |
Rheumatoid Arthritis T cells
|
Organism |
Homo sapiens |
Characteristics |
gender: Female age: 53 tissue: peripheral blood cell type: CD4+ T cells first_diagnosis: Undifferentiated Inflammatory Arthritis working_diagnosis: Rheumatoid Arthritis
|
Growth protocol |
Whole peripheral blood was stored at room temperature for ≤4 hours before processing. After monocyte depletion by immuno-rosetting, an automated magnetic bead-based positive selection protocol was used to isolate CD4+ cells (Stemcell Technologies, Vancouver, Canada) yielding a median cell purity of 98.9%.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
AllPrep DNA/RNA Mini Kit (Qiagen, UK).
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol.
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|
|
Hybridization protocol |
400ng of DNA was bisulphite-converted and hybridised to the Infinium MethylationEPIC BeadChip (Illumina) using standard Illumina protocol.
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Scan protocol |
Illumina iScan.
|
Description |
Freshly isolated and purified peripheral blood T cells
|
Data processing |
Failed probes whose detection p-values were >0.01 in >10% samples were excluded prior to functional normalisation as implemented in the minfi R package, following which additional probes were removed due to; (i) cross-hybridization, (ii) mapping to sex chromosomes, or (iii) presence of a SNP (MAF>0.05) at the interrogated CpG, probe sequence or single base extension site. matrix processed value definition: Normalized average Beta values (Functional normalisation)
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|
|
Submission date |
Sep 17, 2019 |
Last update date |
Dec 21, 2019 |
Contact name |
Arthur Pratt |
E-mail(s) |
arthur.pratt@newcastle.ac.uk
|
Organization name |
Newcastle University
|
Department |
Faculty of Medical Sciences
|
Lab |
Musculoskeletal Research Group
|
Street address |
Framlington Place
|
City |
Newcastle upon Tyne |
ZIP/Postal code |
NE2 4HH |
Country |
United Kingdom |
|
|
Platform ID |
GPL21145 |
Series (2) |
GSE137593 |
Early arthritis CD4+ T cell DNA methylation profiling |
GSE137634 |
Lymphocyte DNA methylation mediates genetic risk at shared immune mediated disease loci |
|