|
| Status |
Public on Sep 02, 2020 |
| Title |
Cell line SKCO1 treated with 1000nM talazoparib |
| Sample type |
RNA |
| |
|
| Source name |
SKCO1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SKCO1
cell type: Colorectal cancer cell
treatment: 1000nM talazoparib
|
| Treatment protocol |
Cells were incubated with 100 and 1000 nM talazoparib (MedChemExpress, #HY-16106) or 0.005% DMSO (Sigma, #D5879) for 48h. After the drug incubation, cells were washed twice with 1xPBS (Gibco, #20010019) and collected after trypsinization.
|
| Growth protocol |
250,000 - 400,000 cells were seeded in 60 mm cell culture dishes (Falcon, #353002) in DMEM/F12 growth media (Thermo Fischer Scientific #31330038) supplemented with 15% Fetal Bovine Serum (Thermo Fischer Scientific #10270106) and 100 U/ml penicillin-streptomycin (Thermo Fischer #15140122). The cells were recovered for 24h before treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cell pellets using the Qiagen Allprep DNA/RNA/miRNA Universal kit, according to the manufacturer's protocol (Qiagen).
|
| Label |
biotin
|
| Label protocol |
RNA was amplified, fragmented and labelled according to the Affymetrix GeneChip HT WT User Guide.
|
| |
|
| Hybridization protocol |
Samples were hybridized onto Affymetrix Human Transcriptome 2.0 Arrays according to the manufacturer's protocol (Affymetrix).
|
| Scan protocol |
Array scanning was performed according to the manufacturer's protocol (Affymetrix).
|
| Description |
SST RMA expression values at the gene-level derived using Expression Console software
|
| Data processing |
Gene-level expression signals were obtained from raw data CEL files by the SST RMA procedure implemented in the Affymetrix Expression Console software.
|
| |
|
| Submission date |
Nov 12, 2019 |
| Last update date |
Sep 02, 2020 |
| Contact name |
Anita Sveen |
| E-mail(s) |
anita.sveen@rr-research.no
|
| Organization name |
Institute for Cancer Research, Oslo University Hospital
|
| Department |
Department of Molecular Oncology
|
| Street address |
P.O.Box 4953, Nydalen
|
| City |
Oslo |
| ZIP/Postal code |
NO-0424 |
| Country |
Norway |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE140258 |
Gene expression profiling of colorectal cancer cell lines after treatment with talazoparib |
|
