|
| Status |
Public on Dec 25, 2019 |
| Title |
Kidney_WT_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
whole Kidney of wildtype mouse, 6 months old
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: wild type
age: 6 months old
tissue: Kidney
|
| Extracted molecule |
total RNA |
| Extraction protocol |
A total of RNA was extracted from the kidneys by Trizol reagent (Invitrogen, CA, USA) as per manufacturer’s instructions followed by RNA purified using the RNeasy mini kit and treated with DNase I. RNA purity and integrity were evaluated by ND-1000 Spectrophotometer (NanoDrop, Wilmington, USA), Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, USA).
|
| Label |
Cy3
|
| Label protocol |
100ng of total RNA from each sample was linearly amplified and labeled with Cy3-dCTP. The labeled cRNAs were purified by RNAeasy Mini Kit (Qiagen). The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000(NanoDrop, Wilmington, USA).
|
| |
|
| Hybridization protocol |
600ng of each labeled cRNA was fragmented by adding 5 μl 10 x blocking agent and 1 μl of 25 x fragmentation buffer, and then heated at 60°C for 30 min. Finally 25 μl 2 x GE hybridization buffer was added to dilute the labeled cRNA. 40μl of hybridization solution was dispensed into the gasket slide and assembled to the Agilent SurePrint G3 Mouse GE 8X60K, V2 Microarrays (Agilent®). The slides were incubated for 17 h at 65°C in an Agilent hybridization oven. then washed at room temperature by using the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology, V 6.5, 2010).
|
| Scan protocol |
The hybridized array was immediately scanned with an Agilent Microarray Scanner D (Agilent Technologies, Inc.)
|
| Description |
114_5_WT
Gene expression profiling of Sting related genes
|
| Data processing |
Microarray results were extracted using Agilent Feature Extraction software v11.0 (Agilent Technologies)
|
| |
|
| Submission date |
Dec 25, 2019 |
| Last update date |
Dec 25, 2019 |
| Contact name |
Arthid Thim-uam |
| E-mail(s) |
arthidth@gmail.com
|
| Phone |
0862162633
|
| Organization name |
Chulalongkorn University
|
| Department |
Biomedical science
|
| Street address |
Rama IV
|
| City |
Bangkok |
| ZIP/Postal code |
10330 |
| Country |
Thailand |
| |
|
| Platform ID |
GPL21163 |
| Series (1) |
| GSE142594 |
Role of Sting in a lupus mouse model of Fcgr2b-deficient mice |
|
