|
| Status |
Public on Jan 24, 2020 |
| Title |
PIR from mouse injected with iCMs, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
heart
|
| Organism |
Mus musculus |
| Characteristics |
tissue: heart PIR
treatment: iCMs
|
| Treatment protocol |
4 weeks post-MI, mice were sacrificed by isofluorane and cervical dislocation. Hearts were explanted and the peri-infarct region was identified via microscopy, dissected out. Samples were rinsed with PBS twice and placed in Trizol (Thermo Fisher Scientific).
|
| Growth protocol |
Female 80-100 day-old SCID mice were purchased from Charles River Laboratories (MA). iCMs were differentiated from iPSCs and cultured until spontaneous contractions were observed. iCM-Ex were precipitated from iCMs.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed using miRNeasy Mini Kit (Qiagen) according to the manufacturers’ instruction.
|
| Label |
biotin
|
| Label protocol |
5.5 μg of sense-strand cDNA was labeled with biotin according to the Clariom S microarray protocol (Thermo Fisher Scientific).
|
| |
|
| Hybridization protocol |
Fragmented and labeled ss-cDNA were hybridized for 16 hr at 45°C on rotation onto Clariom S Mouse Array gene chips (Thermo Fisher Scientific).
|
| Scan protocol |
Arrays were scanned using Affymetrix GeneChip Scanner.
|
| Data processing |
Data were analyzed with Expression Console and Transcription Analysis Console softwares (Thermo Fisher Scientific) using default settings.
|
| |
|
| Submission date |
Jan 23, 2020 |
| Last update date |
Jan 24, 2020 |
| Contact name |
Phillip Yang |
| Organization name |
Stanford University School of Medicine
|
| Street address |
269 Campus Drive, CCSR 3120
|
| City |
Stanford |
| State/province |
California |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE144171 |
Peri-infarct region transcriptome of SCID mice trated with saline control, iPSC-derived cardiomyocytes (iCM), or iCM-derived exosomes (iCM-Ex) |
|
