|
Status |
Public on Sep 30, 2009 |
Title |
volunteer V07 at time point T1 |
Sample type |
RNA |
|
|
Source name |
A PBMC sample from volunteer V07 at time point T1
|
Organism |
Homo sapiens |
Characteristics |
group: Protected cell type: PBMC
|
Treatment protocol |
PBMC samples are collected at various time points during a malaria vaccine trial
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
|
|
Hybridization protocol |
Follow Affymetrix's protocol
|
Scan protocol |
GeneChips were scanned using the GeneArray Scanner
|
Description |
Gene expression data from a volunteer V07 at time point: the day of the third vaccination;it belongs to Protected group
|
Data processing |
CEL files were normalized using RMA method. This dataset was further normalized with a dataset from Plus2.0 Genechip using quantiles normalization method available in the affy package of bioconductor. Probesets that are absent in all samples are eliminated in the later stage of analysis
|
|
|
Submission date |
Sep 29, 2009 |
Last update date |
Sep 29, 2009 |
Contact name |
Maryanne Vahey |
E-mail(s) |
mvahey@hivresearch.org
|
Phone |
301-251-5058
|
Fax |
301-762-7460
|
Organization name |
Walter Reed Army Institute of Research
|
Department |
|
Lab |
Laboratory of Functional Genomics
|
Street address |
1600 East Gude Drive
|
City |
Rockville |
State/province |
MD |
ZIP/Postal code |
20850 |
Country |
USA |
|
|
Platform ID |
GPL571 |
Series (1) |
GSE18323 |
Expression data from a malaria vaccine trial (HG-U133A2.0 and U133 Plus 2.0) |
|