|
| Status |
Public on May 19, 2021 |
| Title |
EHBD-KRAS-adherent-from 3weeks primary tumor |
| Sample type |
RNA |
| |
|
| Source name |
EHBD, KRAS, secondary tumor initiating cells from 3 weeks primary tumor under adherent culture
|
| Organism |
Mus musculus |
| Characteristics |
cell type: secondary EHBD, from 3 weeks primary tumor
culture: adherent culture
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using an RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions.
|
| Label |
Cy3
|
| Label protocol |
Cy3 labeled cRNA were prepared according to the standard Agilent protocol.
|
| |
|
| Hybridization protocol |
The Agilent array were hybridized and washed using the manufacturer's protocol.
|
| Scan protocol |
Agilent arrays were scanned using the Agilent Technologies Scanner G2505C.
|
| Description |
Gene expression data from secondary EHBD tumor initiating cells from 3 weeks primary tumor under adherent culture
|
| Data processing |
The raw data for each spot were normalized by subtraction of the mean background signal intensity determined from the intensities of all blank spots with 95% confidence intervals.
|
| |
|
| Submission date |
May 29, 2020 |
| Last update date |
May 19, 2021 |
| Contact name |
Akiyoshi Kasuga |
| E-mail(s) |
akiyoshi.kasuga@jfcr.or.jp
|
| Phone |
09047116373
|
| Organization name |
Keio University
|
| Street address |
35, Shinanomachi
|
| City |
Shinjuku-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21810 |
| Series (1) |
| GSE151442 |
Comparative gene expression analysis of biliary tract cancer cells under organoid culture and adherent culture. |
|
