|
| Status |
Public on Oct 28, 2009 |
| Title |
liver_1day_500ppm_replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
left laterial lobe of liver, 1 day of dietary dosing, 500 ppm sodium phenobarbital
|
| Organism |
Rattus norvegicus |
| Characteristics |
time point: 1
dose: 500
tissue: liver
lobe: left_lateral
|
| Treatment protocol |
Left lateral lobe of liver was cut into approxiamtely 5mm wide strips; each strip quarted and snap frozen in liquid nitrogen
|
| Growth protocol |
Rats were maintained on CT1 diet and water, ad lib
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted using Qiagen midi RNA extraction kits as per the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Rat Genome 230 v2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip scanner.
|
| Description |
051028_RAC41
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Oct 27, 2009 |
| Last update date |
Oct 27, 2009 |
| Contact name |
Richard Currie |
| E-mail(s) |
richard.currie@syngenta.com
|
| Organization name |
Syngenta
|
| Street address |
Jealott's Hill
|
| City |
Bracknell |
| ZIP/Postal code |
RG42 6EY |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE18753 |
MOLECULAR MECHANISMS ASSOCIATED WITH MARKERS OF HEPATOCARCINOGENICITY DURING A 14 DAY DIETARY STUDY IN MALE FISCHER RAT |
|
