|
| Status |
Public on Jun 04, 2010 |
| Title |
mPFC_SocP60_6 |
| Sample type |
RNA |
| |
|
| Source name |
Brain, fresh dissection immedited following cull
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
tissue: brain (medial prefrontal cortex)
growth protocol: socially-reared
age: postnatal day 60
|
| Treatment protocol |
Half the rats were housed on isolation see Murphy et al., 2010. At the appropriate postnatal age animals were culled and the medial prefrontal cortex dissected rapidly nad snap frozen in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Sample name: cl10120524
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Data filtering criteria linked as supplementary file on Series GSE19653 record.
|
| |
|
| Submission date |
Dec 24, 2009 |
| Last update date |
Jun 03, 2010 |
| Contact name |
Keith J Murphy |
| E-mail(s) |
keith.murphy@ucd.ie
|
| Organization name |
University College Dublin
|
| Department |
School of Biomolecular and Biomedical Science
|
| Lab |
Applied Neurotherapeutics Research Group
|
| Street address |
UCD Conway Institute, Belfield
|
| City |
Dublin |
| ZIP/Postal code |
Dublin4 |
| Country |
Ireland |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE19653 |
Temporal dysregulation of cortical gene expresssion in the isolation-reared Wistar rat |
|
