|
Status |
Public on May 12, 2010 |
Title |
MPCu, Rep 2 |
Sample type |
RNA |
|
|
Source name |
MPC, undifferentiated
|
Organism |
Homo sapiens |
Characteristics |
tissue: skeletal muscle cell type: mesenchymal stem cells differentiation: undifferentiated
|
Treatment protocol |
For adipogenic differentiation, the following protocol was used: 500 ml DMEM F-12 Glutamax, 0.5 mM IBMX, 1 µM dexamethasone, 0.2 mM indomethacin, 10 µg/ ml insulin, 10 % FCS and 1 % PenStrep. For myogenic differentiation, the following protocol was used: 500 ml DMEM F-12 Glutamax, 2 % horse serum and 1 % PenStrep.
|
Growth protocol |
The adipose stem cells, bone marrow mesenchymal stem cells and muscle progenitor cells were cultured in DMEM/F12 Glutamax containing 10% FCS and 1% PenStrep.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using the RNeasy Mini Kit (Qiagen) in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA was prepared with the Illumina TotalPrep RNA Amplification Kit.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
Standard Illumina scanning protocol.
|
Description |
Expression array, undifferentiated muscle progenitor cells.
|
Data processing |
The data were quantile normalised using Illumina BeadStudio software.
The non-normalized data is available in the 'GSE19773_non-normalized.txt' file, which is linked to the Series GSE19773 record as a supplementary file.
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|
|
Submission date |
Jan 06, 2010 |
Last update date |
May 12, 2010 |
Contact name |
Philippe Collas |
Organization name |
University of Oslo
|
Department |
Institute of Basic Medical Sciences
|
Street address |
PO Box 1112 Blindern
|
City |
Oslo |
ZIP/Postal code |
0317 |
Country |
Norway |
|
|
Platform ID |
GPL7363 |
Series (2) |
GSE19773 |
DNA methylation in progenitor cells: expression study |
GSE19795 |
DNA methylation in progenitor cells |
|