|
| Status |
Public on May 12, 2010 |
| Title |
MPCmd, Rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
MPC, myogenic differentiated
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: myogenic-differentiated mesenchymal stem cells
differentiation: in vitro differentiated myocytes
|
| Treatment protocol |
For adipogenic differentiation, the following protocol was used: 500 ml DMEM F-12 Glutamax, 0.5 mM IBMX, 1 µM dexamethasone, 0.2 mM indomethacin, 10 µg/ ml insulin, 10 % FCS and 1 % PenStrep. For myogenic differentiation, the following protocol was used: 500 ml DMEM F-12 Glutamax, 2 % horse serum and 1 % PenStrep.
|
| Growth protocol |
The adipose stem cells, bone marrow mesenchymal stem cells and muscle progenitor cells were cultured in DMEM/F12 Glutamax containing 10% FCS and 1% PenStrep.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNeasy Mini Kit (Qiagen) in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared with the Illumina TotalPrep RNA Amplification Kit.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
Expression array, muscle progenitor cells differentiated under myogenic conditions.
|
| Data processing |
The data were quantile normalised using Illumina BeadStudio software.
The non-normalized data is available in the 'GSE19773_non-normalized.txt' file, which is linked to the Series GSE19773 record as a supplementary file.
|
| |
|
| Submission date |
Jan 06, 2010 |
| Last update date |
May 12, 2010 |
| Contact name |
Philippe Collas |
| Organization name |
University of Oslo
|
| Department |
Institute of Basic Medical Sciences
|
| Street address |
PO Box 1112 Blindern
|
| City |
Oslo |
| ZIP/Postal code |
0317 |
| Country |
Norway |
| |
|
| Platform ID |
GPL7363 |
| Series (2) |
| GSE19773 |
DNA methylation in progenitor cells: expression study |
| GSE19795 |
DNA methylation in progenitor cells |
|
