|
| Status |
Public on Mar 31, 2010 |
| Title |
Preincubated with ALL1 and activated by activated T cell membranes rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Human mast cells HMC-1 preincubated with ALL1 and activated by activated T cell membranes
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HMC-1
|
| Treatment protocol |
Cells were activated for 3 hours via two different activation pathways: A3R activation by Cl-IBMECA, and incubation with activated T cell membranes, with or without 1 hour pretreatment with the peptide ALL1.
|
| Growth protocol |
Human mast cell line HMC-1 were maintained in suspension in supplemented RPMI under standard conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed using the RNeasy Mini Kit (Qiagen Inc. Valencia, CA) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned at the Weizmann Institute Microarray Core Facility using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from HMC-1 cells
|
| Data processing |
The probe set data were generated using Remote Analysis Computation for gene Expression (RACE) via the web interface. The processing algorithm used was gcRMA.
|
| |
|
| Submission date |
Jan 14, 2010 |
| Last update date |
Mar 31, 2010 |
| Contact name |
Dana Baram |
| E-mail(s) |
danabaram1@gmail.com
|
| Organization name |
Tel-Aviv University
|
| Street address |
P.O. Box 39040
|
| City |
Tel-Aviv |
| ZIP/Postal code |
69978 |
| Country |
Israel |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE19888 |
Expression data from HMC-1 mast cells |
|
