|
| Status |
Public on Feb 05, 2010 |
| Title |
wild-type MII-stage oocytes, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
wild-type mouse oocytes collected 16 h post-hCG (human chorionic gonadotropin) injection
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mouse oocyte
strain: C57BL/6
developmental stage: Metaphase II (MII)
genotype: wild-type
|
| Treatment protocol |
Ovarian follicular development was stimulated by intraperitoneal injection of 7.5 IU of PMSG (Calbiochem). Mice were then injected with hCG (7.5IU/mouse), and the oviducts were removed 14-16 h post hCG injection. Ovulated OCCs (oocyte cumulus complexes) were released by gently teasing apart the ampulla of oviducts. Denuded oocytes were obtained by incubating the mass of OCCs in M2 medium containing 0.3 mg/ml of hyaluronidase for about 3-5 min. Oocytes were then collected and washed thoroughly in M2 medium to ensure that no cumulus cells were present.
|
| Growth protocol |
MII oocytes were collected and washed thoroughly in M2 medium (Sigma) to ensure that no cumulus cells were present.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using the Picopure RNA isolation Kit (Arcturus, Mountain View, CA).
|
| Label |
biotin
|
| Label protocol |
Gladstone Genomic Core facility standard protocols were used (http://www.gladstone.ucsf.edu/gladstone/site/genomicscore/section/1914)
|
| |
|
| Hybridization protocol |
Gladstone Genomic Core facility standard protocols were used (http://www.gladstone.ucsf.edu/gladstone/site/genomicscore/section/1914)
|
| Scan protocol |
Gladstone Genomic Core facility standard protocols were used (http://www.gladstone.ucsf.edu/gladstone/site/genomicscore/section/1914)
|
| Description |
none
|
| Data processing |
Data were normalized using the GCRMA algorithm
|
| |
|
| Submission date |
Jan 14, 2010 |
| Last update date |
Feb 04, 2010 |
| Contact name |
Robert Blelloch |
| E-mail(s) |
blellochr@stemcell.ucsf.edu
|
| Phone |
415-476-2838
|
| Organization name |
University of California, San Francisco
|
| Street address |
513 Parnassus Avenue, Campus Box #0525
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE19894 |
MicroRNA function is globally suppressed in mouse oocytes and early embryos |
|
