|
| Status |
Public on Mar 02, 2024 |
| Title |
Serum-NegativeControl-rep1-Phase II |
| Sample type |
protein |
| |
|
| Channel 1 |
| Source name |
Serum,NegativeControl,Phase II
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
age: 30
sampletype: Serum
cancertype: NegativeControl
designphase: Phase II
molecule: Serum autoantibody proteins IgG
|
| Extracted molecule |
protein |
| Extraction protocol |
standard protocol
|
| Label |
Cy3
|
| Label protocol |
After washing with PBST, the microarray was incubated with 1:1000 diluted Fluor conjugated goat anti-human IgG (532nm) and donkey anti-human IgM (635nm) (Jackson ImmunoResearch, West Grove, PA) at RT for 1h in the dark. After washing with PBST, the microarray was rinsed with dd H2O and dried
|
| |
|
| Channel 2 |
| Source name |
Serum,NegativeControl,Phase II
|
| Organism |
Homo sapiens |
| Characteristics |
molecule: Serum autoantibody proteins IgM
|
| Extracted molecule |
protein |
| Extraction protocol |
standard protocol
|
| Label |
Cy5
|
| Label protocol |
After washing with PBST, the microarray was incubated with 1:1000 diluted Fluor conjugated goat anti-human IgG (532nm) and donkey anti-human IgM (635nm) (Jackson ImmunoResearch, West Grove, PA) at RT for 1h in the dark. After washing with PBST, the microarray was rinsed with dd H2O and dried
|
| |
|
| |
| Hybridization protocol |
The microarray was taken out from − 80°C and then incu-bated in blocking buffer (3% BSA in PBS) at RT for 3h.Then a serum sample diluted at 1:200 in binding buffer (1%BSA in PBST) was added to the microarray and incubatedat 4°C overnight
|
| Scan protocol |
The micro-array was scanned with the LuxscanTM 10K-A (CapitalBio Corporation, Beijing, China).
|
| Description |
Phase II of the experimental design
|
| Data processing |
Data analysis was carried out using The GenePix Pro 6.0 (Axon Instruments, Foster City, CA). The signal for each spot (SNR) was defined as the ratio of the fore-ground to the background median intensity and normalized using the median value.
|
| |
|
| Submission date |
Mar 03, 2021 |
| Last update date |
Mar 02, 2024 |
| Contact name |
He Yan Bin |
| E-mail(s) |
heyanbin@bcbio.cn
|
| Phone |
18565610792
|
| Organization name |
BCBIO
|
| Street address |
Sino-European Center A, 2 Lingnan Avenue South, Shunde, Foshan, Guangdong, China
|
| City |
Foshan |
| State/province |
GUANGDONG |
| ZIP/Postal code |
528315 |
| Country |
China |
| |
|
| Platform ID |
GPL29810 |
| Series (1) |
| GSE168198 |
Human serum: Control vs. Lung Cancer |
|
