|
| Status |
Public on Nov 25, 2010 |
| Title |
Klf4 null MEFs Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
MEFs, Klf4 null
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
genotype/variation: Klf4 -/-
cell type: mouse embryonic fibroblast
age: 13 days
|
| Growth protocol |
MEFs were maintained in DMEM, supplemented with 10% FBS, 1% penicillin-streptomycin at 37OC in atomosphere containing 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by DNase I treatment and clean up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Cy3 and Cy5
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Replicate 2 of 3
|
| Data processing |
The data were normalised using quantile normalisation with Genome StudioV1.0.2 (Illumina)
|
| |
|
| Submission date |
May 10, 2010 |
| Last update date |
May 10, 2010 |
| Contact name |
Engda Hagos |
| E-mail(s) |
ehagos@emory.edu
|
| Phone |
404-7125-8415
|
| Fax |
404-727-5767
|
| Organization name |
Emory University
|
| Department |
Digestive Diseases
|
| Lab |
Vincent Yang
|
| Street address |
615 Michael Street
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE21768 |
Klf4 gene disruption is linked to enhanced cellular proliferation and tumor formation |
|
