|
Status |
Public on Oct 01, 2010 |
Title |
Liver_TAK1KO_Rep4 |
Sample type |
RNA |
|
|
Source name |
Liver from 1 year old- TAK1 KO mice
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 age: 1 year old tissue: liver
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNA easy mini kit with on-column DNAseI treatment
|
Label |
Cy3
|
Label protocol |
Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
|
|
|
Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
|
Scan protocol |
Slides were washed and then scanned with an Agilent Scanner.
|
Description |
251486814900_1_3
|
Data processing |
Data were obtained and processed with the Agilent Feature Extraction Software (v9.5). The resulting data were imported into Rosetta Resolver (v7.1) and log2 intensities were exported.
|
|
|
Submission date |
May 19, 2010 |
Last update date |
May 19, 2010 |
Contact name |
NIEHS Microarray Core |
E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
Organization name |
NIEHS
|
Department |
DIR
|
Lab |
Microarray Core
|
Street address |
111 T.W. Alexander Drive
|
City |
RTP |
State/province |
NC |
ZIP/Postal code |
27709 |
Country |
USA |
|
|
Platform ID |
GPL4134 |
Series (1) |
GSE21903 |
Nuclear orphan receptor TAK1/TR4-deficient mice are protected against obesity-linked inflammation, hepatic steatosis, and insulin resistance |
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