|
| Status |
Public on Oct 13, 2021 |
| Title |
CER_40_Case |
| Sample type |
RNA |
| |
|
| Source name |
Human postmortem cerebellum
|
| Organism |
Homo sapiens |
| Characteristics |
aud status: Case
alcoholdailyuse(gram/day): 260
Sex: Male
batch: 4
rin: 7.5
brainweight: 1479
brain.ph: 6.88
pmi: 72
leftrightbrain: Left
causeofdeathcategory: Stroke
smoking: Current
liverclass: Steatosis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the Trizol reagent using the QIAGEN RNeasy mini kit and followed by clean-up and DNase I treatment in accordance with the protocol provided with the kit. Quality control was performed with the Agilent Bioanalyzer System.
|
| Label |
biotin
|
| Label protocol |
About 500 ng of total RNA was used as starting material to obtain labeled cRNA. cRNA was fragmented in the presence of heat and Mg+2 before hybridization to the the Affymetrix Clariom D human array.
|
| |
|
| Hybridization protocol |
A “rotisserie” oven was used to carry out the array hybridization at 45°C and 60 rpm for 16 hours.
|
| Scan protocol |
The images were analyzed using Affymetrix Command Console Viewer
|
| Description |
mRNA & lncRNA transcriptome data
|
| Data processing |
CEL files were processed using the Affymetrix Expression Console (EC) software (v1.4.1)
|
| |
|
| Submission date |
Jul 23, 2021 |
| Last update date |
Oct 13, 2021 |
| Contact name |
Huiping Zhang |
| E-mail(s) |
huipingz@bu.edu
|
| Phone |
6173583689
|
| Organization name |
Boston University School of Medicine
|
| Department |
Psychiatry
|
| Street address |
72 East Concord Street
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02118 |
| Country |
USA |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE180722 |
Profiling mRNA transcriptomic changes using Affymetrix Clarion D assay in 6 regions of postmortem brains of subjects with alcohol use disorder |
|
