|
| Status |
Public on Jun 25, 2010 |
| Title |
CardiacMuscle_Vehicle_168hrs_rep5 |
| Sample type |
RNA |
| |
|
| Source name |
Cardiac muscle sampled 168 hrs post Vehicle Treatment once daily for 14 days
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
gender: male
|
| Treatment protocol |
Cardiac muscle was isolated from the rat, cut sagitally, scored and placed quickly into RNAlater. After 24 hours at 4C, tissues were moved to -80C for storage until all tissues were harvested.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy minikit with Dnase treatment according to manufacturers recommendations
|
| Label |
biotin
|
| Label protocol |
Affymetrix One-Cycle Target Labeling Assay using Affymetrix kit P/N 900493
|
| |
|
| Hybridization protocol |
Standard Affymetrix Protocols
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000
|
| Data processing |
GCOS software using MAS5
|
| |
|
| Submission date |
Jun 24, 2010 |
| Last update date |
Jun 24, 2010 |
| Contact name |
Mary Simcox |
| E-mail(s) |
msimcox@formatherapeutics.com
|
| Phone |
857-209-2853
|
| Organization name |
Forma Therapeutics
|
| Street address |
500 Arsenal St
|
| City |
Watertown |
| State/province |
MA |
| ZIP/Postal code |
02472 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE22553 |
Cardiac Effects of Rosiglitazone in Male Wistar Rats |
|
