|
| Status |
Public on Jul 28, 2010 |
| Title |
DN DC |
| Sample type |
RNA |
| |
|
| Source name |
spleens
|
| Organism |
Mus musculus |
| Characteristics |
strain: Cx3cr1gfp/+ C57BL/6
bmp4 treated: -
dendritic cell: CD4+
|
| Growth protocol |
After collagenase D digestion, spleens from Cx3cr1gfp/+ C57BL/6 mice were enriched for CD11c+ cells by magnetic separation according to the manufacturer’s protocol (MiltenyiBiotec GmbH). Splenic CD11chi cells were isolated using the FACS ARIA high-speed sorter (Becton-Dickinson).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN miniRNA extraction kit was used.
|
| Label |
biotin
|
| Label protocol |
According to Affymetrix manual
|
| |
|
| Hybridization protocol |
According to Affymetrix manual
|
| Scan protocol |
According to Affymetrix manual
|
| Data processing |
MAS5
|
| |
|
| Submission date |
Jul 27, 2010 |
| Last update date |
Apr 10, 2013 |
| Contact name |
Steffen Jung |
| E-mail(s) |
s.jung@weizmann.ac.il
|
| Phone |
0097289342787
|
| Organization name |
The Weizmann Institute of Science
|
| Department |
Immunology and Regenerative Biology
|
| Lab |
Steffen Jung
|
| Street address |
Herzl
|
| City |
REHOVOT |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE23212 |
Gene expression profiling of mouse splenic Dendritic cells subsets |
|
| Relations |
| Reanalyzed by |
GSE45704 |
