|
| Status |
Public on Nov 15, 2010 |
| Title |
liver_Trim28+/+ _rep2 |
| Sample type |
RNA |
| |
|
| Source name |
liver, Trim28+/+
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: FVB/NJ
gender: male
age: 4 weeks
tissue: liver
genotype: Trim28+/+
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the QIAGEN Rneasy-Plus kit in accordance with the prescribed protocol. All RNA samples were checked on Agilent’s Bioanalyzer (Agilent RNA 6000 Nano) and had RNA Integrity Numbers (RINs) greater than 8.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared using the Illumina TotalPrep RNA Amplification Kit (Ambion).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
Illumina GenomeStudio software was used according to the recommendations of the manufacturer. Data was grouped (wildtype versus heterozygotes), background corrected, and normalised (average normalisation). Significantly differentially expressed genes were identified using Illumina's differential expression analysis.
|
| |
|
| Submission date |
Aug 09, 2010 |
| Last update date |
Nov 15, 2010 |
| Contact name |
Suyinn Chong |
| Organization name |
Queensland Institute of Medical Research
|
| Department |
Division of Genetics and Population Health
|
| Lab |
Epigenetics Laboratory
|
| Street address |
300 Herston Rd
|
| City |
Herston |
| State/province |
Queensland |
| ZIP/Postal code |
4006 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE23512 |
Reduced levels of two modifiers of epigenetic gene silencing, Dnmt3a and Trim28, cause increased phenotypic noise. |
|
