|
| Status |
Public on Nov 04, 2010 |
| Title |
breast nontumor part_N_rep1 5425 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
non-tumor part, Buffer 1
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
tissue: non tumor part of breast tissue.
|
| Treatment protocol |
The surgical removed tumors and non-tumor part of breast specimens were excised, collected and snap-frozen in liquid nitrogen immediately before RNA extraction.
|
| Growth protocol |
The fresh samples used for this study were immediately frozen by liquid nitrogen without any additional growth procedure applied before RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent combined with RNAeasy mini kit
|
| Label |
Cy5
|
| Label protocol |
Agilent Low RNA Input Fluorescent Linear Amplification Kit, protocol version 2.0
|
| |
|
| Channel 2 |
| Source name |
Commercially available.
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: Stratagene's human common reference RNA
|
| Treatment protocol |
The surgical removed tumors and non-tumor part of breast specimens were excised, collected and snap-frozen in liquid nitrogen immediately before RNA extraction.
|
| Growth protocol |
The fresh samples used for this study were immediately frozen by liquid nitrogen without any additional growth procedure applied before RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent combined with RNAeasy mini kit
|
| Label |
Cy3
|
| Label protocol |
Agilent Low RNA Input Fluorescent Linear Amplification Kit, protocol version 2.0
|
| |
|
| |
| Hybridization protocol |
Agilent Oligo Microarray Kit, hybridization protocol version 5.0
|
| Scan protocol |
Agilent Oligo Microarray Kit, hybridization protocol version 5.0 with Agilent scanner and Feature Extraction 6.0.
|
| Description |
breast nontumor part_N_rep1
|
| Data processing |
Agilent Two-Color Microarray-Based Gene Expression Analysis, version 4.0. The log2 ratio for each gene was centered by subtracting the median across all samples to discriminate the subclass of the dataset.
|
| |
|
| Submission date |
Sep 14, 2010 |
| Last update date |
Nov 03, 2010 |
| Contact name |
Fon-Jou Hsieh |
| E-mail(s) |
fjhsieh@ntu.edu.tw
|
| Organization name |
National Taiwan University
|
| Department |
Department of obstetrics and gynecology
|
| Street address |
No. 7, Chung-Shan South Rd
|
| City |
Taipei |
| ZIP/Postal code |
100 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL887 |
| Series (1) |
| GSE24124 |
The inferred mechanisms for prognostic features of signal transducer and activator of transcription 3 in an ER(+) breast cancer model system |
|
