|
| Status |
Public on May 18, 2012 |
| Title |
ZNF overexpression - Primary_2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Vector
|
| Organism |
Mus musculus |
| Characteristics |
expression: vector control
cell type: primary mammary epithelial cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit
|
| Label |
Cy3
|
| Label protocol |
Ambion MessageAmp II Amino Allyl kit
|
| |
|
| Channel 2 |
| Source name |
ZNF217
|
| Organism |
Mus musculus |
| Characteristics |
expression: ZNF217 overexpression
cell type: primary mammary epithelial cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit
|
| Label |
Cy5
|
| Label protocol |
Ambion MessageAmp II Amino Allyl kit
|
| |
|
| |
| Hybridization protocol |
Hybridize at 65°C for 14 to 18 hours.
|
| Scan protocol |
Axon Instruments (V1.00) AxImageIO, GenePix 4000B
|
| Data processing |
VALUE is Log (base 2) of the ratio of the median of Channel 2 (usually 635 nm) to Channel 1 (usually 532 nm).
|
| |
|
| Submission date |
Oct 15, 2010 |
| Last update date |
May 18, 2012 |
| Contact name |
Laurie Littlepage |
| E-mail(s) |
laurie.littlepage@ucsf.edu
|
| Phone |
415-476-4758
|
| Organization name |
University of California, San Francisco
|
| Department |
Anatomy
|
| Lab |
Zena Werb
|
| Street address |
513 Parnassus Avenue, Box 0452
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL11062 |
| Series (1) |
| GSE24727 |
The transcription factor ZNF217 is a prognostic biomarker and therapeutic target during breast cancer progression |
|
