|
| Status |
Public on Oct 29, 2010 |
| Title |
DSS_[0-1]_FG86 |
| Sample type |
RNA |
| |
|
| Source name |
Whole blood
|
| Organism |
Homo sapiens |
| Characteristics |
age (y): 9
gender: Female
day of fever relative to defervescence: 0
day of illness: 4
infection: serotype DENV-1
disease state: dengue shock syndrome
patient: FG86
tissue: whole blood
|
| Treatment protocol |
Sample were left at room temperature to thawn
|
| Growth protocol |
Sample were kept in -800C until used
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Paxgene RNA kits (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
Biotinylated amplified cRNA was generated by in vitro transcription (IVT) technology using Illumina® TotalPrep™ RNA Amplification Kit (Ambion, Inc., Austin, TX) according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
850ng of cRNA was hybridized to an Illumina HumanRef-8 V2BeadChip (containing probes to 23,961 RefSeq gene sequences) at 55 °C for 18 hours following the manufacturer’s instructions (Illumina, Inc., San Diego, CA). This was followed by washing, blocking and streptavidin-Cy3 staining steps.
|
| Scan protocol |
The chip was scanned with an Illumina Bead Array Reader confocal scanner
|
| Data processing |
Data was normalised by Beadstudio software
|
| |
|
| Submission date |
Oct 28, 2010 |
| Last update date |
Oct 28, 2010 |
| Contact name |
Long Hoang |
| E-mail(s) |
hoangtruonglong@gmail.com
|
| Organization name |
OUCRU
|
| Street address |
190 Ben Ham Tu
|
| City |
Ho Chi Minh |
| ZIP/Postal code |
848 |
| Country |
Viet Nam |
| |
|
| Platform ID |
GPL6104 |
| Series (1) |
| GSE25001 |
Whole blood gene expression in Vietnamese dengue patients |
|
