|
| Status |
Public on May 04, 2011 |
| Title |
ovarian cancer sample 7 |
| Sample type |
genomic |
| |
|
| Source name |
ovarian cancer sample
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Human ovarian cancer
pfi (months): 47
grading: 1
age (years): 36
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Tumor material was subjected to pathological frozen section; and immediately snaps frozen. 0.5 cm3 of tissue was lysed using Lysing Matrix A (Q-BIOgene) and were homogenized (BIO101/Savant FastPrep Homogenizer). After an overnight incubation with SDS and proteinkinase K, DNA was precipitated by adding ice-cold isopropanol, collected and dried. Concentration and quality of dissolved DNA was measured using Nanodrop Spectrophotometer (Parmacia Biotech).
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Data processing |
BeadStudio software v3.2. Raw data were processed using the BioConductor software package (www.bioconductor.org). Normalization was done by quantile normalization.
|
| |
|
| Submission date |
Oct 29, 2010 |
| Last update date |
May 04, 2011 |
| Contact name |
Qiong Lin |
| E-mail(s) |
qiong.lin@rwth-aachen.de
|
| URL |
http://www.molcell.rwth-aachen.de/
|
| Organization name |
Institute for Biomedical Engineering
|
| Department |
Cell Biology
|
| Street address |
Universitatsklinikum Aachen, RWTH
|
| City |
Aachen |
| State/province |
NRW |
| ZIP/Postal code |
52074 |
| Country |
Germany |
| |
|
| Platform ID |
GPL8490 |
| Series (1) |
| GSE25033 |
Progression Free Survival in Ovarian Cancer is Reflected in Epigenetic DNA-Methylation Profiles |
|
