Extraction of total RNA was performed according to the manufacturer's instructions.
Label
biotin
Label protocol
10 ng of total RNA labeled according to GeneChip WT Pico Kit manufacturer's instructions
Hybridization protocol
Once the labeling reaction was completed, 60uL was combined with the hybridization solution, then hybridized overnight for 17 hours on a Mouse Gene 1.0ST array in an Affymetrix GeneChip Hybridization Oven 640 (Affymetrix, Santa Clara, CA).
Scan protocol
arrays were scanned using the GeneChip Scanner 3000 with 7G upgrade (Thermo Scientific, Wilmington, DE)
Description
Gene expression data from kidney biopsy
Data processing
The image generated was analyzed using the GeneChip Expression Console (Affymetrix, Santa Clara, CA) were .DAT (image), .CEL (cell intensity data), .CHP (probe analysis data) files were generated.
