|
| Status |
Public on Oct 01, 2022 |
| Title |
left_ventricle_wt_old_5 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse heart
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
strain: C57BL/6N
genotype: Wild Type
tissue: left ventricle
age: 21 months
|
| Treatment protocol |
After mouse perfusion with phosphate-buffered saline, hearts were dissected and immediately frozen and then stored at -80°C until processing.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the left ventricles of mice of different age groups using miRNeasy kit (Qiagen) and treated with DNase on column to eliminate genomic contamination, following manufacturer’s instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
9274652021_E
|
| Data processing |
Array data export and quality control were performed with the Genome Studio Software v2011.1 (Illumina). Raw data were imported into the R software v4.0.2 and normalized with the Lumi R/Bioconductor package. Variance stabilizing transformation (VST) was applied and transformed data were then normalized by robust spline normalization (RSN). Probes with a detection P‐value <0.01 in at least 25% of samples were retained.
|
| |
|
| Submission date |
Aug 01, 2022 |
| Last update date |
Oct 01, 2022 |
| Contact name |
Gualtiero Ivanoe Colombo |
| E-mail(s) |
gualtiero.colombo@cardiologicomonzino.it
|
| Phone |
+39 0258002464
|
| Organization name |
Centro Cardiologico Monzino IRCCS
|
| Lab |
Immunology and Functional Genomics
|
| Street address |
Via Carlo Parea, 4
|
| City |
Milano |
| ZIP/Postal code |
20138 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE210192 |
Transcriptomic analysis of cardiac gene expression associated to RAGE deletion and aging |
|
