|
| Status |
Public on May 01, 2014 |
| Title |
Day6 |
| Sample type |
SRA |
| |
|
| Source name |
differentiated human embryonic stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: ES cell line H1
timepoint: Day6
|
| Treatment protocol |
The cells were exposed to the differentiation medium (changed daily) for the duration of differentiation timepoint.
|
| Growth protocol |
H1 human ES cells were grown feeder free in conditioned medium. The cells were passaged every 7 days with a split ratio of 1: 3 - 6.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells using a combination of the trizol protocol and the RNAeasy mini kit (Qiagen, #74106). Poly A RNA was extracted using the Poly (A) Purist Mag kit (Ambion # AM 1992). ABI whole transcriptome library preparation kit (#4425680) was used to generate the RNA-Seq libraries.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
AB SOLiD System 3.0 |
| |
|
| Description |
RHE004
|
| Data processing |
ABI bioscope software was used to align the reads to hg18 assembly and to produce the processed data .bam files.
|
| |
|
| Submission date |
Jan 24, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Paul Robson |
| E-mail(s) |
paul.robson@jax.org
|
| Organization name |
Genome Institute of Singapore
|
| Department |
Developmental biology
|
| Lab |
Dr.Paul Robson's
|
| Street address |
60 Biopolis Street, Genome
|
| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL9442 |
| Series (1) |
| GSE26811 |
Transcriptomics of a novel trophoblast differentiation protocol applied to human embryonic stem cells |
|
| Relations |
| SRA |
SRX039135 |
| BioSample |
SAMN00198547 |
