|
| Status |
Public on Aug 15, 2011 |
| Title |
fresh-frozen tissue_1ep23 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
brain
|
| Organism |
Homo sapiens |
| Characteristics |
gender: m
age: 8
localization: posterior fossa
|
| Treatment protocol |
Fresh frozen material was milled in a Micro-Dismembrator S (B. Braun, Melsungen, Germany).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from biopsy samples using Trizol and RNeasy mini columns following the manufacturer’s instructions.
|
| Label |
Cy3
|
| Label protocol |
Agilent labling protocol following the manufacturer’s instructions.
|
| |
|
| Channel 2 |
| Source name |
5xpool brain
|
| Organism |
Homo sapiens |
| Characteristics |
localization: total brain
|
| Treatment protocol |
Fresh frozen material was milled in a Micro-Dismembrator S (B. Braun, Melsungen, Germany).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from biopsy samples using Trizol and RNeasy mini columns following the manufacturer’s instructions.
|
| Label |
Cy5
|
| Label protocol |
Agilent labling protocol following the manufacturer’s instructions.
|
| |
|
| |
| Hybridization protocol |
Agilent hybridization protocol following the manufacturer’s instructions.
|
| Scan protocol |
Scanned on Agilent Scanner G25505B
|
| Data processing |
Array raw data were generated using Feature Extraction 9.1 Software. Normalization of raw signals was performed using vsn (Huber et al., 2002).
|
| |
|
| Submission date |
Feb 14, 2011 |
| Last update date |
Aug 15, 2011 |
| Contact name |
Hendrik Witt |
| E-mail(s) |
h.witt@dkfz.de
|
| Phone |
+496221424594
|
| Fax |
+496221424639
|
| Organization name |
German Cancer Research Center
|
| Department |
Molecular Genetics
|
| Lab |
Prof. Peter Lichter
|
| Street address |
INF 580
|
| City |
Heidelberg |
| State/province |
BW |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6480 |
| Series (2) |
| GSE27283 |
Human ependymoma samples [expression] |
| GSE27287 |
Delineation of Two Clinically and Molecularly Distinct Subgroups of Posterior Fossa Ependymoma. |
|
