|
Status |
Public on Aug 15, 2011 |
Title |
fresh-frozen tissue_1ep38 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
brain
|
Organism |
Homo sapiens |
Characteristics |
gender: m age: 21 localization: posterior fossa
|
Treatment protocol |
Fresh frozen material was milled in a Micro-Dismembrator S (B. Braun, Melsungen, Germany).
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted from biopsy samples using Trizol and RNeasy mini columns following the manufacturer’s instructions.
|
Label |
Cy3
|
Label protocol |
Agilent labling protocol following the manufacturer’s instructions.
|
|
|
Channel 2 |
Source name |
5xpool brain
|
Organism |
Homo sapiens |
Characteristics |
localization: total brain
|
Treatment protocol |
Fresh frozen material was milled in a Micro-Dismembrator S (B. Braun, Melsungen, Germany).
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted from biopsy samples using Trizol and RNeasy mini columns following the manufacturer’s instructions.
|
Label |
Cy5
|
Label protocol |
Agilent labling protocol following the manufacturer’s instructions.
|
|
|
|
Hybridization protocol |
Agilent hybridization protocol following the manufacturer’s instructions.
|
Scan protocol |
Scanned on Agilent Scanner G25505B
|
Data processing |
Array raw data were generated using Feature Extraction 9.1 Software. Normalization of raw signals was performed using vsn (Huber et al., 2002).
|
|
|
Submission date |
Feb 14, 2011 |
Last update date |
Aug 15, 2011 |
Contact name |
Hendrik Witt |
E-mail(s) |
h.witt@dkfz.de
|
Phone |
+496221424594
|
Fax |
+496221424639
|
Organization name |
German Cancer Research Center
|
Department |
Molecular Genetics
|
Lab |
Prof. Peter Lichter
|
Street address |
INF 580
|
City |
Heidelberg |
State/province |
BW |
ZIP/Postal code |
69120 |
Country |
Germany |
|
|
Platform ID |
GPL6480 |
Series (2) |
GSE27283 |
Human ependymoma samples [expression] |
GSE27287 |
Delineation of Two Clinically and Molecularly Distinct Subgroups of Posterior Fossa Ependymoma. |
|