|
| Status |
Public on Sep 30, 2011 |
| Title |
cervical squamous cell carcinoma cell line_ATCC number HTB_33_24hour timepoint_replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
metastatic site: omentum
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: cervical squamous cell carcinoma cell line
cell line source: American Type Culture Collection (ATCC) number HTB_33
anatomical site: Cervix
gender: Female
osmr expression status: Under
time: 24hour
|
| Treatment protocol |
Cells treated with recombinant OSM (R&D systems) @10 ng/ml added directly to the tissue culture media where appropriate for duration indicated by the time point
|
| Growth protocol |
Cell lines cultured under standard tissue culture conditions - incubated at 37C and in 5% CO2, described in Ng et al 2007, PMID: 17516585
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep kit for Illumina arrays
|
| Label |
biotin
|
| Label protocol |
Standard Illumina hybridisation protocol
|
| |
|
| Hybridization protocol |
Standard Illumina scanning protocol
|
| Scan protocol |
biotinylated cRNA were prepared with the Ambion TotalPrep kit for Illumina arrays
|
| Description |
ME180-T24-B
cervical squamous cell carcinoma cell line obtained from the American Type Culture Conditions (ATCC) ATCC number HTB_33_24hour timepoint_replicate2
Replicate 2
|
| Data processing |
Raw data were filtered to remove any non detected probe (detection pvalue above 0.01 for all samples) and then normalised using quantile normalisation. The software used is the lumi package from bioconductor (R).
|
| |
|
| Submission date |
Feb 24, 2011 |
| Last update date |
Sep 30, 2011 |
| Contact name |
Julien Bauer |
| E-mail(s) |
jb393@cam.ac.uk
|
| Organization name |
Cambridge University
|
| Street address |
Tennis court road
|
| City |
Cambridge |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB2 1QP |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6102 |
| Series (1) |
| GSE27480 |
Gene-expression analysis of Oncostatin-M (OSM) signalling in cervical squamous cell carcinomas over-expressing the Oncostatin-M receptor (OSMR) |
|
