|
| Status |
Public on Mar 22, 2011 |
| Title |
Sclera week 1, biological rep5 |
| Sample type |
RNA |
| |
|
| Source name |
Sclera tissue from week 1 mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain: Balb/cJ
age: Week 1
|
| Growth protocol |
Balb/cJ mice (Mus musculus) were obtained from the animal holding unit of the National University of Singapore.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from single cryogenically ground mouse sclera (n=30, 6 samples each from week 1-, 2-, 3-, 6-and 8-old mice) using MELTTM Total Nucleic Acid Isolation System (Ambion Inc, Austin, TX) according to the manufacturer’s instructions
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA using Genechip® Whole Transcript Sense Target Labeling Assay and (Affymetrix, Inc, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on a Mouse Gene 1.0 ST array(Affymetrix, Inc, Santa Clara, CA). GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using Genechip® Hybridzation kit(Affymetrix, Inc, Santa Clara, CA).
|
| Scan protocol |
GeneChips were scanned using Genechip® Scanner 3000 7G (Affrymetrix, Inc, Santa Clara, CA).
|
| Description |
Gene expression data from week 1 sclera
|
| Data processing |
The data were analyzed with Partek Genomics Suite version 6.5 beta and normalized using Genechip robust multiarray averaging (GC-RMA) and batch removal tool.
|
| |
|
| Submission date |
Mar 21, 2011 |
| Last update date |
Mar 22, 2011 |
| Contact name |
Wan'e Lim |
| E-mail(s) |
lim.wan.e@seri.com.sg
|
| Phone |
+65 63224568
|
| Fax |
+65 63224599
|
| Organization name |
Singapore Eye Research Institute
|
| Street address |
11 Third Hospital Ave
|
| City |
Singapore |
| ZIP/Postal code |
168751 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE28056 |
Gene expression profiling of mouse sclera during post-natal development |
|
