|
| Status |
Public on Nov 22, 2023 |
| Title |
Model2 miRNA-seq |
| Sample type |
SRA |
| |
|
| Source name |
lens capsules
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: lens capsules
treatment: Na2SeO3 is injected subcutaneously
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TRIzol reagent (ThermoFisher, USA)
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
The data from RNA-Seq were first filtered (Cutadapt and Fqtrim) and assessed for quality (FastQC), and then compared to the reference genome (miRDeep and HISAT2).
The data source for the reference genome is Ensembl database (http://asia.ensembl.org)
Supplementary files format and content: excel;gene count
|
| |
|
| Submission date |
Feb 28, 2023 |
| Last update date |
Nov 22, 2023 |
| Contact name |
Li Xinhui |
| E-mail(s) |
lixh257@mail2.sysu.edu.cn
|
| Organization name |
Sun Yat-sen university
|
| Street address |
Guangzhou
|
| City |
Guangzhou |
| ZIP/Postal code |
510275 |
| Country |
China |
| |
|
| Platform ID |
GPL25947 |
| Series (2) |
| GSE226272 |
Transcriptome sequencing and microRNA–mRNA regulatory network construction in the lens from a Na2Se03-induced Sprague Dawley rat cataract model [miRNA] |
| GSE226274 |
Transcriptome sequencing and microRNA–mRNA regulatory network construction in the lens from a Na2Se03-induced Sprague Dawley rat cataract model |
|
| Relations |
| BioSample |
SAMN33481823 |
| SRA |
SRX19522209 |
