|
| Status |
Public on Mar 18, 2023 |
| Title |
Control_1 |
| Sample type |
SRA |
| |
|
| Source name |
Lens
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Lens
genotype: Celf1lacZKI/+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Standard total RNA isolation protocol by RNeasy mini kit (Qiagen)
Standard protocol for Illumina sequencing
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Control
|
| Data processing |
Sequence reads were trimmed to remove adaptor sequence
Trimmed reads were map to the mouse genome (GRCm38.p6) with STAR software (2.7.8a)
Uniquely mapped reads were count by featureCount (v2.0.0)
Supplementary files format and content: GRCm38.p6
Supplementary files format and content: Differential expression analysis by edgr, with cpm (count per millions) normaliezd for each sample and statistical Gene Expression difference for each genes.
|
| |
|
| Submission date |
Mar 14, 2023 |
| Last update date |
Oct 13, 2023 |
| Contact name |
Salil Anil Lachke |
| E-mail(s) |
salil@udel.edu
|
| Organization name |
University of Delaware
|
| Department |
Biological Sciences
|
| Street address |
351 Wolf Hall, University of Delaware
|
| City |
Newark |
| State/province |
Delaware |
| ZIP/Postal code |
19716 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE227293 |
High-throughput transcriptomics of Celf1 conditional knockout lens identifies downstream networks linked to cataract pathology |
|
| Relations |
| BioSample |
SAMN33750320 |
| SRA |
SRX19668391 |
