|
| Status |
Public on Oct 04, 2011 |
| Title |
cleavage inhibition control #20 (4068264018 F) |
| Sample type |
RNA |
| |
|
| Source name |
2-cell stage
|
| Organism |
Mus musculus |
| Characteristics |
cell type: 2-cell stage
|
| Treatment protocol |
The genome of the mouse zygote or the mouse blastomere was exchanged at mitosis. The extent of reprogramming was determined during the following cell cycle.
|
| Growth protocol |
mouse embryos were cultured in KSOM
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PicoPure RNA isolation kit (Arcturus)
|
| Label |
biotin
|
| Label protocol |
Biotin label introduced according to protocols of the Illumina Total Prep RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Hybridization according to Illumina protocols on Illumina Sentrix Mouse Expression BeadChip RefSeq 8 v1.1 (GPL6103) or Mouse Expression BeadChip® RefSeq 8 v2.0 (GPL6885) as indicated. Arrays are hybridized, washed, blocked and stained with streptavidin-Cy3. The protocol is WGGEX Direct Hybridization Assay Guide (11322355 A).
|
| Scan protocol |
According to Illumina protocols with Illumina iScan. Protocols can be found on the illumina web page.
|
| Description |
1-cell embryo at 2-cell timing (cleavage inhibition), , approx. 22-24h post first mitosis
|
| Data processing |
Data were normalized to average signal, background was subtracted using Illumina GenomeStudio. There are two series presented in a SuperSeries. One series was generated with GPL6103, the other with GPL6885. Normalized data are presented only for probes present in both GPL6103 AND GPL6885. Hybridization duplicates were not used for data analysis, but only for quality control.
|
| |
|
| Submission date |
Apr 22, 2011 |
| Last update date |
Oct 04, 2011 |
| Contact name |
Dieter Egli |
| E-mail(s) |
degli@nyscf.org
|
| Phone |
617 4968659
|
| Organization name |
The New York Stem Cell Foundation Research Institute
|
| Department |
NYSCF
|
| Lab |
Egli
|
| Street address |
3960 Broadway
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL6103 |
| Series (1) |
| GSE28811 |
Reprogramming is achieved within a single cell cycle after mouse nuclear transfer |
|
