|
Status |
Public on Feb 21, 2024 |
Title |
A699-62 |
Sample type |
RNA |
|
|
Source name |
liver
|
Organism |
Homo sapiens |
Characteristics |
tissue: liver patient: pat 16 disease: CRC Met steatosis: <5% time point: 1
|
Extracted molecule |
total RNA |
Extraction protocol |
not provided
|
Label |
Biotin
|
Label protocol |
Affymetrix GeneChip® 3’ IVT Express Kit (P/N 702646 Rev. ; Cat.No. 901228
|
|
|
Hybridization protocol |
Affymetrix GeneChip Human Genome U219 Array Plate (Cat No. ???); Affymetrix GeneChip® HT hybridization, Wash and Stain Kit (Cat No. 900720); Affymetrix Genechip® fluidics station 450: protocol FS450_0001 for Cartridge Arrays
|
Scan protocol |
Affymetrix Scanner: GCS3000
|
Description |
Pat16 <5% CRC Met t1 cDNA, from amplified total RNA
|
Data processing |
Affymetrix Geneexpression Console (Expression Console EC1.2.1.20) using Affymetrix default analysis settings for generation of CEL-files. Data Pre-Processing and Filtering (Partek Software, v.6.6): RMA (background correction, quantile normalization across all chips in the experiment, log2 transformation, median polish summarization); paired t-test
|
|
|
Submission date |
Apr 03, 2023 |
Last update date |
Feb 21, 2024 |
Contact name |
Silvia Groiss |
Organization name |
Medical University of Graz
|
Department |
Institute of Pathology
|
Street address |
Neue Stiftingtalstraße 6
|
City |
Graz |
ZIP/Postal code |
8010 |
Country |
Austria |
|
|
Platform ID |
GPL13667 |
Series (2) |
GSE228782 |
Inter-patient heterogeneity in the hepatic ischemia-reperfusion injury transcriptome: implications for research and diagnostics [MUG data] |
GSE228783 |
Inter-patient heterogeneity in the hepatic ischemia-reperfusion injury transcriptome: implications for research and diagnostics |
|