|
Status |
Public on Oct 10, 2012 |
Title |
WU-BC4, mouse passage3 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Stratagene Human Universal Reference spiked with MCF7 and ME16C mRNA
|
Organism |
Homo sapiens |
Characteristics |
reference: Stratagene Human Reference total RNA from 10 human cell lines: 1_Adenocarcinoma, mammary gland 2_Hepatoblastoma, liver 3_Adenocarcinoma, cervix 4_Embryonal carcinoma, testis 5_Glioblastoma, brain 6_Melanoma 7_Liposarcoma 8_Histiocytic Lymphoma; macrophage; histocyte 9_ Lymphoblastic leukemia, T lymphoblast 10_Plasmacytoma; myeloma; B lymphocyte. Also, mRNA spiked in from MCF7 and ME16C.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extractions followed by Dnase treatment and column purification.
|
Label |
Cy3
|
Label protocol |
Agilent Low Input Linear Amplification Kit and protocol
|
|
|
Channel 2 |
Source name |
primary breast tumor, WU-BC4, xenograft passage3
|
Organisms |
Homo sapiens; Mus musculus |
Characteristics |
tissue type: primary breast tumor sample type: oct block from human:mouse xenograft which contained at least 50% tumor cellularity clinical er: Negative clinical pgr: Negative clinical her2: Negative
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extractions followed by Dnase treatment and column purification.
|
Label |
cy5
|
Label protocol |
Agilent Low Input Linear Amplification Kit and protocol
|
|
|
|
Hybridization protocol |
Agilent 60-mer oligo microarray processing protocol
|
Scan protocol |
Microarrays were scanned with an Agilent B Scanner using 5um, single pass scanning mode and eXtended dynamic range.
|
Description |
PAM50 Basal Subtype
|
Data processing |
txt files and tif images generated by Agilent's Feature Extraction, v9.5.3.1, were uploaded into the UNC Microarray database. Final loess, normalized log2 ratios (Cy5-sample/Cy3-control) for each probe were retrieved.
|
|
|
Submission date |
Apr 26, 2011 |
Last update date |
Dec 26, 2023 |
Contact name |
Cynthia Ma |
E-mail(s) |
cynthiaxma@wustl.edu
|
Phone |
3143628903
|
Organization name |
Washington University
|
Department |
Medical Oncology
|
Lab |
Dr. Cynthia Ma Lab
|
Street address |
4515 Mckinley, 4515 Mckinley
|
City |
st louis |
State/province |
Missouri |
ZIP/Postal code |
63627 |
Country |
USA |
|
|
Platform ID |
GPL6480 |
Series (2) |
GSE28860 |
WU-BC 3, 4, and 5 PAM50 Subtypes with Passage and Gene Expression Comparison Between Xenografts and Primary Tumors |
GSE41685 |
WHIM Gene Expression Data_Update Through WHIM 26 |
|
Relations |
Reanalyzed by |
GSM4486586 |