|
| Status |
Public on Sep 15, 2023 |
| Title |
e17.5N: non-α cell_3 |
| Sample type |
SRA |
| |
|
| Source name |
pancreas
|
| Organism |
Mus musculus |
| Characteristics |
tissue: pancreas
genotype: Gcg-Timer
time: E17.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Rneasy mini plus kit (Qiagen).
RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
gene_expression e17.5 GcgTimer.xlsx:
Genedata Profiler Genome v10.1.14b
Sequence reads were aligned and mapped to GRCm38/mm10 using STAR v2.5.2b and Genedata Profiler Genome.
Read count extraction and normalization were performed using Genedata Profiler Genome.
Assembly: GRCm38/mm10
Supplementary files format and content: Excel files include TPM values and raw gene counts for each sample.
*********************
gene_expression e17.5 GcgTimer_IRES-EGFP-mRFP added.xlsx:
DRAGEN Bio-IT Platform (3.7.5).
Sequence reads were aligned and mapped to genome GRCm39 and sequence of EGFP, mRFP1 and IRES by using DRAGEN Bio-IT Platform.
Read count extraction and normalization were performed using DRAGEN Bio-IT Platform.
Assembly: GRCm39/mm39
Supplementary files format and content: Excel files include TPM values and raw gene counts for each sample.
|
| |
|
| Submission date |
Apr 05, 2023 |
| Last update date |
Sep 15, 2023 |
| Contact name |
MIWA HIMURO |
| Organization name |
Juntendo University Graduate School of Medicine
|
| Department |
Department of Metabolism and Endocrinology
|
| Street address |
Hongo 2-1-1, Bunkyo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
1138421 |
| Country |
Japan |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE229090 |
Novel time-resolved reporter mouse reveals spatial and transcriptional heterogeneity during alpha cell differentiation |
|
| Relations |
| BioSample |
SAMN34086225 |
| SRA |
SRX19888861 |
