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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 15, 2023 |
| Title |
Novel time-resolved reporter mouse reveals spatial and transcriptional heterogeneity during alpha cell differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Glucagon-expressing pancreatic α cells have attracted much attention for their plasticity to transdifferentiate into insulin-producing β cells; however, it remains unclear precisely when and where α cells emerge from and what regulates α-cell fate. We therefore explored the spatial and transcriptional heterogeneity of α-cell differentiation by a novel time-resolved reporter system. We established the mouse model, ‘Gcg-Timer’, in which newly generated α cells can be distinguished from more differentiated cells by their fluorescence. Transcriptome analysis of Gcg-Timer embryos revealed that the mRNAs related to angiogenesis were enriched in newly generated-α cells. Novel time-resolved analysis with Gcg-Timer reporter mouse uncovered spatiotemporal features of α-cell neogenesis, which will enhance our understanding of cellular identity and plasticity within the islets.
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| Overall design |
This study characterizes the transcriptomes of mouse pancreatic α-lineage cells. Pancreas from Gcg-Timer at E17.5 were dissociated and sorted by FACS into 3 groups (N: non-α cell, A: green dominant_newly generated-α cell, and B: green and red_more differentiated-α cell). 4 replicate data were colleced from different mother mice. RNA were extracted and RNA sequencing was performed.
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| Contributor(s) |
Himuro M, Wakabayashi Y, Taguchi T, Katahira T, Suzuki L, Iida H, Ogihara T, Nishida Y, Sasaki S, Lynn FC, Hiraoka Y, Oshima S, Okamoto R, Fujitani Y, Watada H, Miyatsuka T |
| Citation(s) |
37870650 |
| Submission date |
Apr 05, 2023 |
| Last update date |
Dec 15, 2023 |
| Contact name |
MIWA HIMURO |
| Organization name |
Juntendo University Graduate School of Medicine
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| Department |
Department of Metabolism and Endocrinology
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| Street address |
Hongo 2-1-1, Bunkyo-ku
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| City |
Tokyo |
| ZIP/Postal code |
1138421 |
| Country |
Japan |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| GSM7152364 |
e17.5N: non-α cell_4 |
| GSM7152365 |
e17.5A: green dominant_newly generated-α cell_1 |
| GSM7152366 |
e17.5A: green dominant_newly generated-α cell_2 |
| GSM7152367 |
e17.5A: green dominant_newly generated-α cell_3 |
| GSM7152368 |
e17.5A: green dominant_newly generated-α cell_4 |
| GSM7152369 |
e17.5B: green and red_more diffrentiated-α cell_1 |
| GSM7152370 |
e17.5B: green and red_more diffrentiated-α cell_2 |
| GSM7152371 |
e17.5B: green and red_more diffrentiated-α cell_3 |
| GSM7152372 |
e17.5B: green and red_more diffrentiated-α cell_4 |
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| Relations |
| BioProject |
PRJNA952797 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE229090_gene_expression_e17.5_GcgTimer.xlsx |
15.0 Mb |
(ftp)(http) |
XLSX |
| GSE229090_gene_expression_e17.5_GcgTimer_IRES-EGFP-mRFP_added.xlsx |
12.2 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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