|
| Status |
Public on Oct 03, 2023 |
| Title |
Anesthesia with sevoflurane |
| Sample type |
SRA |
| |
|
| Source name |
liver
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: liver
genotype: wild type
treatment: Subjection of anesthesis with sevoflurane for 6h
time: 6 hours
|
| Treatment protocol |
Rats were subjected to inhalational anesthesia using either sevoflurane (4.5%) or desflurane (6%) and rats were sacrificed by decapitation immediately after their loss of consciousness or after subjection of inhalational anesthesia for 6 hours and then portions of their livers were recovered and immersed in RNAlater until RNA preparation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were harvested using RNeasy Midi Kit (Qiagen).
RNA libraries for RNA-seq were prepared using TruSeq Stranded Total RNA Library Prep Gold Kit (illumina) according to manufacturer’s instruction.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Terminal
Sequence reads were trimmed for adaptor sequence/low-quality sequence using sickle (parameter- -q 30 -l 20 )
Trimmed sequence reads were mapped to rn6 using hisat2 and samtools
Read count extraction and normalization were performed using Stringtie
Assembly: rn6
Supplementary files format and content: tab-delimited text files include RPKM and TPM values for each Sample
|
| |
|
| Submission date |
Apr 23, 2023 |
| Last update date |
Oct 03, 2023 |
| Contact name |
Akihiko Okuda |
| E-mail(s) |
akiokuda@saitama-med.ac.jp
|
| Phone |
81-42-984-4787
|
| Organization name |
Saitama Medical University
|
| Department |
Division of Biomedical Sciences, Research Center for Genomic Medicine
|
| Street address |
1397-1 Yamane
|
| City |
Hidaka |
| State/province |
Saitama |
| ZIP/Postal code |
3501241 |
| Country |
Japan |
| |
|
| Platform ID |
GPL25947 |
| Series (1) |
| GSE230365 |
Comparison of global gene expression profile associated with inhalation anesthesia between sevoflurane and desflurane |
|
| Relations |
| BioSample |
SAMN34331753 |
| SRA |
SRX20069171 |
