|
| Status |
Public on Jun 17, 2011 |
| Title |
CD8- cDC rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Spleen
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/B6
cell type: CD8- cDC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from splenic cells was prepared with an Qiagen RNeasy mini or micro kit . RNA quality was ascertained using the Agilent Bioanalyser 2100 with the NanoChip protocol.
|
| Label |
Cy3
|
| Label protocol |
125 ng of RNA was labelled with biotin using the Total Prep RNA amplification kit (Ambion).
|
| |
|
| Hybridization protocol |
Labeled RNA was prepared for hybridization to Illumina Sentrix Mouse-6 v2 Beadchips following standard Illumina protocol. The bead arrays were washed and blocked in E1 buffer 10 min in rocking incubator and 10 additional minutes with 2 ml of E1 buffer. They were then stained with streptavidin conjugated to Cy3 fluorescent dye. The fluorescent reagent was washed away with E1BC solution. The arrays were finally air dried.
|
| Scan protocol |
Arrays were sanned by an Illumina BeadArray Reader (Illumina California USA). Default setting was used.
|
| Data processing |
Un-normalized summary probe profiles were output from BeadStudio and analysed using the limma packages of the Bioconductor open-source software project (http://www.bioconductor.org). The raw intensities were normexp background corrected with offset 16, normalized by neqc function.
|
| |
|
| Submission date |
Jun 16, 2011 |
| Last update date |
Jun 17, 2011 |
| Contact name |
Priyanka Sathe |
| E-mail(s) |
sathe@wehi.edu.au
|
| Organization name |
The Walter and Eliza Hall Institute of Medical Research
|
| Street address |
1G Royal Parade
|
| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3050 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE30022 |
Gene expression profiles of mouse splenic macrophages and CD8+ and CD8- conventional dendritic cells |
|
