|
Status |
Public on Aug 28, 2011 |
Title |
3H |
Sample type |
RNA |
|
|
Source name |
3-Unsorted
|
Organism |
Homo sapiens |
Characteristics |
sample #: 3 cell source: AML leukemia stem cells (lsc): N/A sorted fraction: Unsorted
|
Growth protocol |
Standard protocol for primary human tissue
|
Extracted molecule |
total RNA |
Extraction protocol |
TriZol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
RNA was amplified using the Ovation Biotin RNA Amplification and Labeling System (NuGEN, San Carlos, CA). Biotinylated cRNA were prepared according to the standard Affymetrix protocol from Nugen amplified RNA.
|
|
|
Hybridization protocol |
The cDNA was fragmented, labeled and hybridized to Affymetrix HT_HG-U133A microarrays (Affymetrix, Santa Clara, CA)
|
Scan protocol |
standard Affymetrix protocol
|
Data processing |
Data was normalized by robust multichip average using GeneSpring GX (Agilent).
|
|
|
Submission date |
Jul 01, 2011 |
Last update date |
Oct 20, 2011 |
Contact name |
Kolja Eppert |
Organization name |
University Health Network
|
Street address |
101 College St., rm 8-301
|
City |
Toronto |
State/province |
Ontario |
ZIP/Postal code |
M5G 1L7 |
Country |
Canada |
|
|
Platform ID |
GPL3921 |
Series (2) |
GSE30375 |
Gene expression data from sorted and unsorted primary human acute myeloid leukemia (AML) samples |
GSE30377 |
Human Hematopoietic and Leukemic Stem Cell Gene Expression Profiles |
|