|
| Status |
Public on Aug 28, 2011 |
| Title |
8C |
| Sample type |
RNA |
| |
|
| Source name |
8-CD34+/CD38+
|
| Organism |
Homo sapiens |
| Characteristics |
sample #: 8
cell source: AML
leukemia stem cells (lsc): LSC-
sorted fraction: CD34+/CD38+
|
| Growth protocol |
Standard protocol for primary human tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriZol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
RNA was amplified using the Ovation Biotin RNA Amplification and Labeling System (NuGEN, San Carlos, CA). Biotinylated cRNA were prepared according to the standard Affymetrix protocol from Nugen amplified RNA.
|
| |
|
| Hybridization protocol |
The cDNA was fragmented, labeled and hybridized to Affymetrix HT_HG-U133A microarrays (Affymetrix, Santa Clara, CA)
|
| Scan protocol |
standard Affymetrix protocol
|
| Data processing |
Data was normalized by robust multichip average using GeneSpring GX (Agilent).
|
| |
|
| Submission date |
Jul 01, 2011 |
| Last update date |
Oct 20, 2011 |
| Contact name |
Kolja Eppert |
| Organization name |
University Health Network
|
| Street address |
101 College St., rm 8-301
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL3921 |
| Series (2) |
| GSE30375 |
Gene expression data from sorted and unsorted primary human acute myeloid leukemia (AML) samples |
| GSE30377 |
Human Hematopoietic and Leukemic Stem Cell Gene Expression Profiles |
|
