|
| Status |
Public on Dec 30, 2012 |
| Title |
Hypothalamus tissue at day 42, biological replication ID J4 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Hypothalamus tissue after 42 days of caloric restriction
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
gender: Male
tissue: Hypothalamus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted according to the manufacturer instructions.
|
| Label |
biotin
|
| Label protocol |
RNA was labeled according to the manufacturer instructions.
|
| |
|
| Hybridization protocol |
RNA was hybridized to Affymetrix array according to the manufacturer instructions.
|
| Scan protocol |
GeneChip mouse genome 430 2.0 microarrays were scanned according to the manufacturer instructions.
|
| Data processing |
Raw data were background subtracted and quantile normalized using Rosetta Resolver software
|
| |
|
| Submission date |
Jul 08, 2011 |
| Last update date |
Dec 30, 2012 |
| Contact name |
Zhi Wang |
| E-mail(s) |
zhi.wang@sagebase.org
|
| Organization name |
Sage Bionetworks
|
| Street address |
1100 Fairview Ave N, M1-C108
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98115 |
| Country |
USA |
| |
|
| Platform ID |
GPL7368 |
| Series (1) |
| GSE30534 |
Expression of obese mice subjected to diet induced weight loss for 60 days |
|
