|
| Status |
Public on Aug 02, 2023 |
| Title |
306_1_S93, silk gland_rep 2 |
| Sample type |
SRA |
| |
|
| Source name |
Silk gland
|
| Organism |
Bombyx mori |
| Characteristics |
tissue: Silk gland
strain: A306
silk gland weight phenotype_1: 0.732 g
silk gland weight phenotype_2: 0.656 g
|
| Growth protocol |
Silkworms of each strain were fed at the same regulated feeding conditions (25℃ and 71% humidity) up to day 5 of the fifth larval instar.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was extracted from the silk gland using TRIzol (Invitrogen, Carlsbad, CA, USA). 1 µg of total RNA was used for the construction of sequencing libaries.
The RNA libraries were validated according to the manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
306_2
|
| Data processing |
The original data were analyzed using fastp version 0.19.5, and clean data with Q30 > 92.93% was obtained from each sample
Compare clean data(reads) with reference genome.
Use assembly software StringTie Version 1.3.3b for reference-based assembly.
The differentially expressed genes were determined at |log2 (fold change)| > 1 and p-adjust < 0.05 using DESeq2 Version 1.24.0
Assembly: StringTie
Supplementary files format and content: tab-delimited text file includes raw counts for each Sample
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample
|
| |
|
| Submission date |
Jul 26, 2023 |
| Last update date |
Aug 02, 2023 |
| Contact name |
Sun Lindan |
| E-mail(s) |
sunlindan@ujs.edu.cn
|
| Phone |
13675118671
|
| Organization name |
Jiangsu University
|
| Department |
School of Life and Sciences
|
| Street address |
301 xuefu road
|
| City |
Zhenjiang |
| ZIP/Postal code |
212013 |
| Country |
China |
| |
|
| Platform ID |
GPL23127 |
| Series (1) |
| GSE239380 |
Identification of genes associated with the development of silk glands using transcriptomics in silkworm (Bombyx mori) |
|
| Relations |
| BioSample |
SAMN36719188 |
| SRA |
SRX21167735 |
