|
Status |
Public on Apr 17, 2024 |
Title |
WT D15-D H3K9me3 Replicate 3 |
Sample type |
SRA |
|
|
Source name |
dorsally patterned neuronal precursors
|
Organism |
Homo sapiens |
Characteristics |
cell type: dorsally patterned neuronal precursors stem cell_source: H9 hESC passage: p10-p60 timepoint: D15-D antibody: H3K9me3
|
Growth protocol |
Embryonic stem cells were mainatined in mTeSR media on Vitronectin coated cultureware.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was processed using CUT&Tag (Epicypher) and isolated using phenol-chloroform follow by the NucleoSpin® Gel and PCR Clean-Up kit (Macherey-Nagel). After PCR amplification samples with unique dual-end indexes were pooled in equimolar concentrations.
|
|
|
Library strategy |
OTHER |
Library source |
genomic |
Library selection |
other |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Raw reads were processed using AIAP (v1.1) for trimming, alignment, bigWig generation and peak calling Assembly: hg38 Supplementary files format and content: bigWig density file Supplementary files format and content: narrowPeak or broadPeak file containing output from MACS2 peakcalling Library strategy: CUT&Tag
|
|
|
Submission date |
Jul 27, 2023 |
Last update date |
Apr 17, 2024 |
Contact name |
Kristen Kroll |
Organization name |
Washington University School of Medicine
|
Department |
Developmental Biology
|
Lab |
Kristen Kroll
|
Street address |
320 McDonnell Sciences/660 S. Euclid Ave.
|
City |
Saint Louis |
State/province |
MO |
ZIP/Postal code |
63110 |
Country |
USA |
|
|
Platform ID |
GPL24676 |
Series (2) |
GSE239400 |
Assessment of chromatin state changes during human cortical interneuron development |
GSE239481 |
Defining Cis-regulatory Elements and Transcription Factors that Control Human Cortical Interneuron Development |
|
Relations |
BioSample |
SAMN36728833 |
SRA |
SRX21172210 |